Search results for "Brevibacterium flavum"
showing 2 items of 2 documents
Effect of changes in the cellular energy state on glucose transport activity in Brevibacterium flavum
2000
Abstract The effect of changes in cellular energy state on 6[14C]glucose uptake and activity of the phosphoenolpyruvate:glucose phosphotransferase system (PTS) in Brevibacterium flavum RC 115 cells was investigated. Energy generation in cells was varied by adding an inhibitor of cellular respiration (potassium cyanide) and an uncoupler of oxidative phosphorylation (pentachlorophenol) to the cell culture as well as by changing thiamine concentration in the bacterial growth medium. The results showed that glucose uptake in ‘respiring’ cells was inversely correlated with bacterial respiration activity: uptake declined with excess ATP generation and increased with lower ATP synthesis caused by …
Metabolism and Lysine Biosynthesis Control in Brevibacterium Flavum: Impact of Stringent Response in Bacterial Cells
2005
Parameters affecting lysine biosynthesis by Brevibacterium flavum RC 115 cells under stringent response conditions (test guanosine 5′-diphosphate 3′-diphosphate accumulation in cells) caused by threonine limitation were investigated. Experimental results confirmed that an increase in lysine biosynthesis by this bacterium under stringent response conditions might be a result of an increase in the intracellular concentration of NADPH as well as lysine export activity.